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Endogenous miR-451a expression was upregulated time-dependently during neuronal differentiation of <t>NT2</t> cells ( A ). ( B-F) show neuronal microscopic images of NT2 cells during retinoic acid-induced differentiation in vitro. The statistical significance of changes in miR-451a expression was tested using Friedman‘s test. *p≤0.05 after Bonferroni correction. n = 4 biological replicates; Scale bars: 100 μm. Error bars show standard error of the mean (SEM).
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Endogenous miR-451a expression was upregulated time-dependently during neuronal differentiation of <t>NT2</t> cells ( A ). ( B-F) show neuronal microscopic images of NT2 cells during retinoic acid-induced differentiation in vitro. The statistical significance of changes in miR-451a expression was tested using Friedman‘s test. *p≤0.05 after Bonferroni correction. n = 4 biological replicates; Scale bars: 100 μm. Error bars show standard error of the mean (SEM).
Human Ntera2 D1 Teratocarcinoma Nt2 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ntera2 nt2 human teratocarcinoma cells  (ATCC)
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Endogenous miR-451a expression was upregulated time-dependently during neuronal differentiation of <t>NT2</t> cells ( A ). ( B-F) show neuronal microscopic images of NT2 cells during retinoic acid-induced differentiation in vitro. The statistical significance of changes in miR-451a expression was tested using Friedman‘s test. *p≤0.05 after Bonferroni correction. n = 4 biological replicates; Scale bars: 100 μm. Error bars show standard error of the mean (SEM).
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Endogenous miR-451a expression was upregulated time-dependently during neuronal differentiation of NT2 cells ( A ). ( B-F) show neuronal microscopic images of NT2 cells during retinoic acid-induced differentiation in vitro. The statistical significance of changes in miR-451a expression was tested using Friedman‘s test. *p≤0.05 after Bonferroni correction. n = 4 biological replicates; Scale bars: 100 μm. Error bars show standard error of the mean (SEM).

Journal: PLoS ONE

Article Title: MicroRNA-451a overexpression induces accelerated neuronal differentiation of Ntera2/D1 cells and ablation affects neurogenesis in microRNA-451a -/- mice

doi: 10.1371/journal.pone.0207575

Figure Lengend Snippet: Endogenous miR-451a expression was upregulated time-dependently during neuronal differentiation of NT2 cells ( A ). ( B-F) show neuronal microscopic images of NT2 cells during retinoic acid-induced differentiation in vitro. The statistical significance of changes in miR-451a expression was tested using Friedman‘s test. *p≤0.05 after Bonferroni correction. n = 4 biological replicates; Scale bars: 100 μm. Error bars show standard error of the mean (SEM).

Article Snippet: Ntera2/D1 (NT2) (ATCC, LCG standards) and HEK293T cells (human embryonic kidney cells, provided by Dr Alexander Deutsch, Division of Haematology, Medical University Graz) were maintained in high glucose Dulbecco’s modified Eagle Medium (DMEM, Sigma-Aldrich) containing 10% Fetal bovine serum (FBS, Sigma-Aldrich), 1% Non-Essential Amino Acids (NEAA, Sigma-Aldrich) and 1% Penicillin-Streptomycin (Sigma-Aldrich) at 5% CO 2 and 37°C.

Techniques: Expressing, In Vitro